Adaptive optics multiphoton microscopy to study ex vivo ocular tissues

We develop an adaptive optics (AO) multiphoton microscope by incorporating a deformable mirror and a Hartmann-Shack wavefront sensor. The AO module operating in closed-loop is used to correct for the aberrations of the illumination laser beam. This increases the efficiency of the nonlinear processes in reducing tissue photodamage, improves contrast, and enhances lateral resolution in images of nonstained ocular tissuesThis work has been supported by Ministerio de Educacion´ y Ciencia, Spain (Grants FIS2007-64765 & Consolider SAUUL CSD2007-00033) and Fundacion Seneca, Murcia, Spain (Grant No. 04524/GERM/06). The authors thank J.Postprint (published version