Cerebrospinal Fluid Real-Time Quaking-Induced Conversion Is a Robust and Reliable Test for Sporadic Creutzfeldt-Jakob Disease: An International Study
- McGuire, LI
- Poleggi, A
- Poggiolini, I
- Suardi, S
- Grznarova, K
- Shi, S
- de Vil, B
- Sarros, S
- Satoh, K
- Cheng, K
- Cramm, M
- Fairfoul, G
- Schmitz, M
- Zerr, I
- Cras, P
- Equestre, M
- Tagliavini, F
- Atarashi, R
- Knox, D
- Collins, S
- Haik, S
- Parchi, P
- Pocchiari, M
- Green, A
DOIAbstract
Real-time quaking-induced conversion (RT-QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt-Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT-QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160-165
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