Integrated genome analysis suggests that most conserved non-coding sequences are regulatory factor binding sites
- Hemberg, M
- Gray, JM
- Cloonan, N
- Kuersten, S
- Grimmond, S
- Greenberg, ME
- Kreiman, G
DOIAbstract
More than 98% of a typical vertebrate genome does not code for proteins. Although non-coding regions are sprinkled with short (<200 bp) islands of evolutionarily conserved sequences, the function of most of these unannotated conserved islands remains unknown. One possibility is that unannotated conserved islands could encode non-coding RNAs (ncRNAs); alternatively, unannotated conserved islands could serve as promoter-distal regulatory factor binding sites (RFBSs) like enhancers. Here we assess these possibilities by comparing unannotated conserved islands in the human and mouse genomes to transcribed regions and to RFBSs, relying on a detailed case study of one human and one mouse cell type. We define transcribed regions by applying a nove...
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