A procedure has been developed to isolate dendritic cells to a high degree of purity from fresh blood. Prior enrichment methods have relied upon an initial 1-2-d culture period. Purified fresh isolates lack the characteristic morphology, phenotype, and immunostimulatory function of dendritic cells. The purified cells have the appearance of medium sized lymphocytes and express substantial levels of CD4, but lack the T cell molecules CD3, CD8, and T cell receptor. When placed in culture, the cells mature in a manner resembling the previously described, cytokine-dependent maturation of epidermal dendritic cells (Langerhans cells). The cells enlarge and exhibit many cell processes, express much higher levels of major histocompatibility complex ...
Background and Objectives. It has been previously demonstrated that dendritic cells (DCs) are charac...
Dendritic cells (DCs) are effective as antigen-presenting cells in the immune system and are present...
We examined the effects of different cytokine combinations and culture conditions on the expansion a...
A procedure has been developed to isolate dendritic cells to a high degree of purity from fresh bloo...
The life history of dendritic cells (DC) is now established from their origins from bone marrow stem...
Freudenthal, P.S., and Steinman, R.M. The distinct surface of human blood dendritic cells as observe...
Dendritic cells (DC) are highly efficient antigen-presenting cells that initiate the primary immune ...
Dendritic cells (DC) are potent APC that can be purified from cultured peripheral blood non-T cells....
Introduction: Dendritic cells have a high potential in presentation of antigens and can be generated...
Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they ori...
Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they ori...
Prior studies have identified a subset of dendritic cells in human blood, as well as their stimulato...
Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We...
Currently, immune cell-based therapies, particularly those that utilize dendritic cells (DCs), are a...
The functional properties of dendritic cells (DCs) are strictly dependent on their maturational stat...
Background and Objectives. It has been previously demonstrated that dendritic cells (DCs) are charac...
Dendritic cells (DCs) are effective as antigen-presenting cells in the immune system and are present...
We examined the effects of different cytokine combinations and culture conditions on the expansion a...
A procedure has been developed to isolate dendritic cells to a high degree of purity from fresh bloo...
The life history of dendritic cells (DC) is now established from their origins from bone marrow stem...
Freudenthal, P.S., and Steinman, R.M. The distinct surface of human blood dendritic cells as observe...
Dendritic cells (DC) are highly efficient antigen-presenting cells that initiate the primary immune ...
Dendritic cells (DC) are potent APC that can be purified from cultured peripheral blood non-T cells....
Introduction: Dendritic cells have a high potential in presentation of antigens and can be generated...
Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they ori...
Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they ori...
Prior studies have identified a subset of dendritic cells in human blood, as well as their stimulato...
Studies on purified blood dendritic cells (DCs) are hampered by poor viability in tissue culture. We...
Currently, immune cell-based therapies, particularly those that utilize dendritic cells (DCs), are a...
The functional properties of dendritic cells (DCs) are strictly dependent on their maturational stat...
Background and Objectives. It has been previously demonstrated that dendritic cells (DCs) are charac...
Dendritic cells (DCs) are effective as antigen-presenting cells in the immune system and are present...
We examined the effects of different cytokine combinations and culture conditions on the expansion a...