Add to ORKGHydrolase catalyzed reactions are used for selective chemical catalysis. With directed evolution, rational mutations and molecular modeling the selectivity of these hydrolases can be increased and the origin of selectivity determined. This study investigates four selective hydrolase catalyzed reactions. Using molecular modeling the unusual regioselectivity of Pseudomonas cepacia lipase (PCL) and the selectivity of Candida antarctica B lipase (CAL-B) in nucleoside acylation reactions has been attributed to the binding of the nucleoside base within the active site. The enantio-selectivity of Pseudomonas fluorescens esterase (PFE) has been improved using a rational approach to directed evolution and models to explain the origin of imp...
Background: The biocatalytic production of enantiopure compounds is of steadily increasing importanc...
Biocatalysis can be applied in organic synthetic chemistry to counter challenges posed by increased ...
The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conven...
Researchers use enzymes for enantio- and regioselective reactions because of their high selectivity...
The introduction of enantiopure active substances is enforced through stricter regulations of the US...
Occasionally, researchers need to modify enzymes through amino acid substitutions to make them more ...
Pseudomonas cepacia lipase (PCL) is a highly enantioselective catalyst in the hydrolysis or formati...
On the basis of the X-ray crystal structure of the lipase from Pseudomonas cepacia (PcL), an enzyme ...
Chiral compounds are of steadily increasing importance to the chemical industry, in particular for t...
Two serine hydrolases, Candida antarctica Lipase B (CALB) and para-nitrobenzyl (Bs2) esterase from B...
Candida antarctica lipase B (CALB) is a robust and easily expressed enzyme used widely in academic a...
Chiral compounds are of steadily increasing importance to the chemical industry, in particular for t...
Although chemists often exploit the high stereo selectivity of hydrolytic enzymes to produce pure en...
In organic synthesis, lipases are the most frequently used biocatalysts. They are efficient stereose...
In organic synthesis, lipases are the most frequently used biocatalysts. They are efficient stereose...
Background: The biocatalytic production of enantiopure compounds is of steadily increasing importanc...
Biocatalysis can be applied in organic synthetic chemistry to counter challenges posed by increased ...
The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conven...
Researchers use enzymes for enantio- and regioselective reactions because of their high selectivity...
The introduction of enantiopure active substances is enforced through stricter regulations of the US...
Occasionally, researchers need to modify enzymes through amino acid substitutions to make them more ...
Pseudomonas cepacia lipase (PCL) is a highly enantioselective catalyst in the hydrolysis or formati...
On the basis of the X-ray crystal structure of the lipase from Pseudomonas cepacia (PcL), an enzyme ...
Chiral compounds are of steadily increasing importance to the chemical industry, in particular for t...
Two serine hydrolases, Candida antarctica Lipase B (CALB) and para-nitrobenzyl (Bs2) esterase from B...
Candida antarctica lipase B (CALB) is a robust and easily expressed enzyme used widely in academic a...
Chiral compounds are of steadily increasing importance to the chemical industry, in particular for t...
Although chemists often exploit the high stereo selectivity of hydrolytic enzymes to produce pure en...
In organic synthesis, lipases are the most frequently used biocatalysts. They are efficient stereose...
In organic synthesis, lipases are the most frequently used biocatalysts. They are efficient stereose...
Background: The biocatalytic production of enantiopure compounds is of steadily increasing importanc...
Biocatalysis can be applied in organic synthetic chemistry to counter challenges posed by increased ...
The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conven...