Effects of the protein phosphatase inhibitors calyculin A and okadaic acid at the cholinergic nerve terminal

  • Issa, Amalia Mary.
Publication date
January 1998
Publisher
McGill University

Abstract

The aim of these studies was to characterize the involvement of the serine/threonine phosphatases 1 and 2A in cholinergic nerve terminal function. This was studied in rat hippocampal formation using the specific inhibitors of phosphatases I and 2A, calyculin A (Cal A) and okadaic acid (OKA).The first set of experiments demonstrated that treatment with OKA or Cal A reduced tissue ACh content, choline acetyltransferase (ChAT) activity, choline uptake and [3H] ACh synthesis in rat hippocampal formation, but spontaneous ACh release was not affected. Thus, it is clear that phosphatase activity plays a role in presynaptic central cholinergic nerve terminal function, particularly in the regulation of ACh synthesis. The mechanisms that regulate ACh...

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