Thylakoid organization and photosystem distribution in Coleochaete scutata : further homologies between charophytes and higher plants

Thylakoid organization and the distribution of photosystem (PS) I and II proteins in the green alga Coleochaete scutata were analyzed by electron microscopy. Thylakoids were observed to associate in varied conformations. Extended bands of thylakoids were present, as in other algae, but numerous grana, characteristic of higher plants, were also detected. Immunolabelling experiments were conducted with two heterologous antisera raised against PS proteins in the cyanobacterium Synechococcus elongatus: one antiserurn against the 60 and 62 kDa PSI reaction centre proteins, the other against the 47 kDa PSII core antenna protein. PSI was 2.6 times more concentrated in the nonappressed membranes (NAM) than in the appressed (AM), with 62% of labelling on NAM. The concentration of PSII in AM was 1.6 times that of the NAM, accounting for 75% of PSII. Thus, in C scutata, PSI and PSII are located in both appressed and nonappressed thylakoid membranes, but with a trend towards the lateral heterogeneity of PS proteins observed in higher plants. These results support the body of ultrastructural and molecular data linking charophytes with the ancestry of higher plants