Temporal and morphologic sequence of DNA replication in the mammalian chromosome complements.

Almost a decade ago, Breuer and Pavan (1955) reported for the first time that during larval growth DNA synthesis proceeds disproportionately along polytene chromosomes of Rhynochosciara (R. angelae). In such chromosomes, treated by Feulgen reaction, the intensity of stain appeared heavier at the points where the bulbs were formed, thus indicating localized accumulation of DNA molecules. [...