Studies on in vitro antibody production; the effect of a virus infection on antibody synthesis.

Since the advent of improved tissue culture techniques the study of animal viruses has made rapid progress. The effect of a virus infection on the host cells has been studied from many aspects. One aspect is the alteration of the protein synthetic capacities of the infected cells. Many studies have been performed on the proteins of the virus particle such as the antigens and the haemagglutinins, or the proteins of the cells, such as the enzymes used to synthesize the virus particles. Little attention has been paid to the cellular proteins which were not involved in the synthesis of the virus particle. Such a protein is antibody. The following report shows how the metbod was establisbed for the quantitation of antibody produced in vitro. It will examine the effect of Newcastle disease virus on the immunologically competent cells in spleen cell suspensions. [...