Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinases) that comprises an active catalytic domain and several C-terminal domains. We compare the binding affinity and association rate constants of the N- terminal domain form of wild-type tissue inhibitor of metalloproteinase (TIMP-3; N-TIMP-3) and its mutants against full-length recombinant TACE and the truncated form of its catalytic domain. We show that the C-terminal domains of TACE substantially weaken the inhibitory action of N-TIMP-3. Further probing with hydroxamate inhibitors indicates that both forms of TACE have similar active site configurations. Our findings highlight the potential role of the C-terminal domains of ADAM proteinases i...
Cells direct the functions and activities of their thousands of proteins through several mechanisms....
Tissue inhibitors of metalloproteinases (TIMPs) are the endogenous modulators of the zinc-dependent ...
TNF-α converting enzyme (TACE/ADAM-17) is a type I membrane-bound metalloproteinase that processes t...
Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinase...
TACE is a disintegrin metalloproteinase that processes tumor necrosis factor-α and a host of other m...
We previously reported that tumor necrosis factor-α converting enzyme (TACE) was specifically inhibi...
AbstractTNF-α converting enzyme (TACE; ADAM-17) is a membrane-bound disintegrin metalloproteinase th...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-...
TNF-α converting enzyme (TACE; ADAM-17) is a membrane-bound disintegrin metalloproteinase that proce...
Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a dual inhibitor of the matrix metalloproteinase...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-...
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the rele...
Tumour necrosis factor-α (TNF-α) converting enzyme (TACE) is a membrane-anchored, multiple-domain zi...
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the rele...
We investigated whether the affinity of tissue inhibitor of metalloproteinases (TIMP)-3 for adamalys...
Cells direct the functions and activities of their thousands of proteins through several mechanisms....
Tissue inhibitors of metalloproteinases (TIMPs) are the endogenous modulators of the zinc-dependent ...
TNF-α converting enzyme (TACE/ADAM-17) is a type I membrane-bound metalloproteinase that processes t...
Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinase...
TACE is a disintegrin metalloproteinase that processes tumor necrosis factor-α and a host of other m...
We previously reported that tumor necrosis factor-α converting enzyme (TACE) was specifically inhibi...
AbstractTNF-α converting enzyme (TACE; ADAM-17) is a membrane-bound disintegrin metalloproteinase th...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-...
TNF-α converting enzyme (TACE; ADAM-17) is a membrane-bound disintegrin metalloproteinase that proce...
Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a dual inhibitor of the matrix metalloproteinase...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-...
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the rele...
Tumour necrosis factor-α (TNF-α) converting enzyme (TACE) is a membrane-anchored, multiple-domain zi...
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the rele...
We investigated whether the affinity of tissue inhibitor of metalloproteinases (TIMP)-3 for adamalys...
Cells direct the functions and activities of their thousands of proteins through several mechanisms....
Tissue inhibitors of metalloproteinases (TIMPs) are the endogenous modulators of the zinc-dependent ...
TNF-α converting enzyme (TACE/ADAM-17) is a type I membrane-bound metalloproteinase that processes t...