Study on the Determination of Inorganic Anions in Water by Reversed Phase HPLC

本文试验反相高效液相色谱法分离分析水中氯离子、硝酸根和硫酸根的条件,研究可能干扰、提高选择性和提高灵敏度的方法,建立应用于自来水和海水等实际样品的分离分析方法。A study on the determination of Cl---, NO_3--(-) and SO_4--(2-) in tap water and sea water by reversed phase ion pair HPLC was proposed.The separation of inorganic anions was accomplished on a RP-C_(18) column using ion pair chromatographic technique and the UV detector was used to detected anions at 320 nm by indirect photometric detection method.The mobile phase (pH5.0) composed of 2.0mmol/L tetrabutylammonium hydroxide (ion pair reagent) and 2.0 mmol/L Salicylic acid (visualization agent)in methanol-water (2: 98).Good separation and determination results were obtained.The detection limit (ng) of anions was as Follows: Cl---, 3.4; NO_3---, 25.8; SO4_--(2-), 9.6