Preparation and evaluation of hydroxycamptothecin-loaded poly (D,L-lactic acid) nanoparticles using dialysis method

透析法制备聚合物缓释纳米粒，工艺简单并且可以避免使用表面活性剂，易于工业化生产。因此，受到人们越来越多的关注。 羟基喜树碱(HCPT)是从中国特有的珙桐科植物喜树中分离出的一种吲哚类生物碱，它能通过抑制拓扑异构酶Ⅰ而抑制肿瘤细胞的增殖，对非小细胞肺癌、腹水型肝癌、胃癌、及白血病有一定疗效，为广谱抗肿瘤药物。临床上主要剂型为注射用水针剂的开环羧酸盐HCPT，因其临床疗效低、半衰期短等缺点限制了该制剂的应用。为了提高该药的生物利用率，减小毒副作用，本文以D，L-PLA为药物载体，以闭环HCPT为模型药物，采用直接透析法制备载羟基喜树碱聚乳酸纳米粒，并考察其体外释放性能、细胞毒性及体内抑瘤效果。 ...Dialysis method was developed to prepare polymer particles in recent years. Dialysis method is an acceptably simple and effective method for preparation particles without the additional surfactants, which is expected to apply in industrial production. 10-hydroxycamptothecin (HCPT) is a promising broad-spectrum antitumor agent that targets the nuclear enzyme topoisomerase I and has achieved remar...学位：理学硕士院系专业：医学院药学系_药物化学学号：2452007115254