Investigation on phenotype of pterygial epithelial progenitor cells

目的：探讨人翼状胬肉上皮祖细胞的表型及其在体外克隆培养时发生的异常增殖及分化。 方法：选择20例（27眼）原发性翼状胬肉患者，应用免疫荧光及免疫组织化学染色法检测27例翼状胬肉上皮细胞中ABCG2、IPO13、K15、K14、P63和K19、Pax6、MUC5AC蛋白的表达，并与正常结膜、角膜缘上皮对照。将10例翼状胬肉和正常结膜组织标本的上皮细胞层分离，用胰蛋白酶消化后接种在3T3细胞滋养层上，于SHEM培养基中培养12天后，对形成的克隆进行计数、拍照和统计分析计算克隆形成率，应用ABCG2、IPO13、P63、Pax6的免疫荧光及组织化学染色判断翼状胬肉上皮克隆中干细胞及增殖指标的表达情...Purpose: To investigate the distribution and density of stem cell associated markers in the pterygial epithelium and proliferative capacity as well as abnormal differentiation of pterygial epithelial stem cells in clone culture model in vitro. Methods: Twenty patients (Twenty-seven eyes) with primary pterygium, who underwent surgical excision, were enrolled in this study. Immunohistochemistry st...学位：医学硕士院系专业：医学院临床医学系_肿瘤学学号：2452007115423