Isolation of microsatellite markers from Porphyra haitanensis and their application in genetic diversity of Porphyra

坛紫菜是一种营养价值很高的海藻，在福建省有悠久的栽培历史。运用分子生物学技术分析坛紫菜种质资源的遗传多样性，寻找相关的分子标记，可以指导培育实践，加快育种进程。本实验首先构建了部分坛紫菜小片段基因组文库，从中筛选到1500左右个阳性克隆，在215个阳性克隆中筛选到4个微卫星位点。选择其中的2个微卫星位点ATN4和GAN5以及从GeneBank里搜索的江蓠的3个微卫星位点AJ512812、AJ512806和AJ512804对9个紫菜样品（LYG、RD、CS、GL1、DXY、BBD、JJS、NH和LSD）进行PCR扩增。5个微卫星位点共检测到31个DNA条带，其中条带最多的位点是AJ512812，...Porphyra haitanensis is seaweeds of high nutiitive value, and it has a long history of culture in Fujian province. In this thesis, we first constructed a small-size genomic library of P.haitanensis and got 1500 positive recombinants.Among which we obtained 4 microsatellite sequences from 215 positive clones.Choosing 2 of the microsatellite loci in P.haitanensis and 3 microsatellite loci of ...学位：理学硕士院系专业：生命科学学院生物学系_动物学学号：20012600