Degradation characteristics of dimethyl isophthalate and formaldehyde by two marine fungi

苯二甲酸酯（PAEs）作为塑料产品中的增塑剂已被发现具有内分泌干扰毒性。随着塑料工业的发展，这类物质对海洋生态环境造成巨大的威胁。在之前的研究中，我们从近海红树林沉积物中富集分离到1株PAEs的降解真菌Fusariumsp.DMT-5-3，其对不同同分异构体的邻苯二甲酸二甲酯（DMPEs）的降解途径有很大的差异，暗示其产生的DMPEs降解酯酶具有很高的底物选择性。本论文以间苯二甲酸二甲酯（DMI）为底物，诱导Fusariumsp.DMT-5-3产酶，通过离子交换层析、分子筛凝胶层析等方法分离纯化到一个DMI的降解酯酶蛋白。纯化的DMI酯酶蛋白分子量约76kDa，是由两个相同亚基组成的二聚体蛋白...Phthalate esters (PAEs) are used as plasticizers in plastic products and these compounds have been identified as toxic pollutants due to their endocrine-disrupting activity. With the rapid development of plastic industry, PAEs may pose serious threat in marine ecosystem. In our previous study, a marine fungus Fusarium sp. DMT-5-3 capable of degrading PAEs was isolated from mangrove sediments by en...学位：理学硕士院系专业：生命科学学院_微生物学学号：2162011115232