Determination of 1-hydroxy pyrene in fish bile by synchronous fluorescence spectrometry

A method for the determination of 1-hydroxy pyrene in fish bile by synchronous fluorescence spectrometry has been established. The experimental results shown that 1-hydroxy pyrene, one of the most imporant polynuclear aromatic hydrocarbons (PAHs) metabolites in fish bile, can be determined directly by synchronous fluorescence spectrometry. Fish bile samples were obtained form Pagrosomus major and Nibea miichthys, which were sampled at three different sampling stations in Xiamen marine cultural area. The usefulness of 1-hydroxy pyrene analytical method in fish bile as a biomonitoring tool for PAHs pollution is discussed