Immunocytochemical studies on the optic ganglia of the mud crab, Scylla serrata (Forskl)

【中文摘要】 采用兔抗哺乳类血清,应用链霉菌抗生物素蛋白-过氧化物酶免疫细胞化学技术,观察了锯缘青蟹视神经节中5 羟色胺、胰高血糖素、神经肽Y和生长抑素免疫阳性细胞和神经纤维的形态和分布,结果发现,视神经节的4个神经髓均有5 羟色胺免疫阳性细胞,除X器检出1个较大型阳性细胞外,其余均为小型细胞.视外髓、视内髓和视端髓都具有胰高血糖素免疫阳性细胞,视端髓的神经髓质阳性染色深,X器中阳性细胞成群分布,窦腺免疫阳性反应强.神经肽Y免疫阳性细胞在视神经层和视外髓为小型阳性细胞,在视内髓和视端髓检出较大型阳性细胞,另有小型阳性细胞散布于X器中.生长抑素免疫阳性细胞分布于4个神经髓,数量少. 4种免疫阳性物的特异性分布模式,可为其不同的神经生理作用提供形态学证据. 【英文摘要】 s: Immunocytochemistry technologies were applied to observe the immunoreactive neurons and neuropils of serotonin (5-HT), glucagon (Glu), neuropeptide Y (NPY) and somatostatin (SOM) in the optic ganglia of Scylla serrata. The results are as follows: 5-HT-immunoreactive cells are located in lamina ganglionaris(LG), medulla externa(ME), medulla interna(MI)and medulla terminalis(MT). Except one large immunoreactive cell in the X organ, the other immunoreactive cells are small. The presence of Glu-immunoreactiv...福建省重中之重资助项目; 高等学校博士学科点专项科研基金资助项目(20010384010);国家自然科学基金资助项目(30300269)