Lecba defektu chrupavek velkych kloubu autologni a alogenni transplantaci chondrograftu.

A method of a chondral defect treatment is presented. It consists of preparing a suitable medium - chondrograft - and its testing in an animal experiment. A full-thickness defect of articular cartilage was made in the load-bearing area of medial condyle of pig's femur. The knee of this animal and the human knee are similar. The study was performed in 16 pigs using both animals' knees. The chodrograft was prepared from chondrocytes mixed with the fibrin glue Tissucol (Baxter, Germany). According to the type of chondrocytes an autologous or allogenic chondrograft was prepared. The allogenic chondrograft was placed into the area of the defect in the left knee and 21 days later the autologous chondrograft was implanted into the defect in the right knee of the same animal. This procedure was done in 13 animal, 6 defects (3 animals) were left without treatment as a negative control group. The evaluation of the chondrograft's quality consisted of gross inspection and histological investigation. The results of grafts incorporation were evaluated by dissection, light and electron microscopy. All defect treated by autologous and allogenic chondrografts were healed in by a hyaline-like cartilage. No significant statistical difference in the results of defect's healing by autologous and allogenic chondrografts was found. The chondrografts based on the fibrin glue Tissucol seem to be an acceptable medium for acute defects of articular cartilage in load-bearing areas defects healing.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi