Analyza DNA chromozomu Y.

At least 50% case of sterile couples are caused by a male factor. In men with azoospermia or severe oligozoospermia, a connection with microdeletions in the region AZF on the chromosome Y was found. AZF is divided into three non-overlapping regions - AZFa, AZFb and AZFc. The main candidate genes are as follows: UPS9Y and DBY in AZFa, RBMY in AZFb and DAZ in AZFc. In males with AZF deletions, testicular histology varies considerably, from total absence of germ cells through maturation arrest to hypospermatogenesis. Correlation between localization of deletions in AZFa, AZFb, AZFc and histological finding was not confirmed. Y-deleted spermatozoa can transmit the defect on male offspring (holandric heredity from father to son), either by a natural way or especially due to assisted reproduction. But most Y-chromosome microdeletions arise de novo. This work aimed at implementation of DNA examination of infertile men in the AZF region. The objectives were as follows: to choose appropriate loci for AZF examination and to optimize PCR conditions; to examine the AZF region in infertile men; to find criteria for DNA screening in infertile men; to suggest clinical measures for men with AZF microdeletions.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi