Ucinky laserovych mikromanipulaci na chovani bunky.

Pulse laser was used because it permits microdestructive processes to occur in the cell. Blepharisma and Paramecium cells responded to its destructive action in a different way, as demonstrated by vidoe recording. While Paramecium cells showed marked contraction of wound edges, Blepharisma reacted in a less pronounced way. This can be due to a different organization of the cortical cytoskeleton. In addition, Blepharisma cells contain blepharismin, a specific red pigment, that is probably responsible for a higher absorption of laser light that produces more serious damage. Blepharisma cells may survive without detectable damage even after destruction of the whole apical end. In these experiments we found out that the damage caused by laser stimulates cell proliferation. Our experiments provided evidence that micromanipulations and destructive changes facilitated by laser tweezers have a wide scope of application in cell biology, such as studies of mechanical properties of cytoskeletal structures or spatial arrangement of the cell interior, with a possibility of mapping distribution of its individual cell components.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi