Estudo de marcadores bioquimicos e celulares da rizogenese adventicia em sobreiro e videira micropropagados

De novo formation of adventitious root meristems involves complex changes in the plant developmental process, creating one of the main difficulties to the cloning of woody species. Before the first cell divisions and formation of the root meristem, numerous endogenous factors interact at biochemical and cellular level. The study of these precocious metabolic changes can lead to the identification of cellular and biochemical markers and allow to understand the basic rooting mechanisms, improving the rooting rates, especially in woody plants. In micro propagated cork oak and grapevine induced to root with auxin for 4-5 days, we have analysed some biochemical and cellular parameters that potentially can function as markers for adventitious rhizogenesis, namely free and conjugated polyamines and free and conjugated auxins. We have also studied the association between rooting and other parameters such as "tissue printing" for aminoacid patterns and cyclin expression levels. Our results seem to point "tissue printing for H_2O_2" as a simple and specific test, able to function as a precocious marker for adventitious rooting in grapevine. In cork oak, besides the variation of histological patterns during rhizogenesis, endogenous levels of IAA and of the polyamine putrescine showed evident differences between induced plants and controlsAvailable from Fundacao para a Ciencia e a Tecnologia, Servico de Informacao e Documentacao, Av. D. Carlos I, 126, 1249-074 Lisboa, Portugal / FCT - Fundação para o Ciência e a TecnologiaSIGLEPTPortuga