NEW SITE-SPECIFIC ENDONUCLEASES FROM STRAINS BACILLIUS STEAROTHERMOPHILUS M6, BACILLUS SPECIES KT5 AND BACILLUS SPECIES KT6

The work covers the enzymes of modification-restriction from the thermophilic microorganisms. The aim of the work is to search the producers of the site-specific endonucleases and methylases among natural isolats of the thermophilic bacteria and to study the extracted enzymes. The site-specific endonucleases have been discovered firstly in the cells of B. stearothermophilus M6 and B. species KT5. The collection of 67 strains of the thermophilic bacteria has been created. The strain-producer of the endonuclease with new specifics (B. species KT61) has been found. The endonucleases BstM61, BspKT51 and BspKT61 are the new reagents at study of the DNA structure and new tools for construction of the recombinant DNA. The obtained results can find application in the works of the Institute of Protein, Russian Academy of Sciences, Institute of Molecular Biology Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio