ANTISTRICTIVE PROTEINS CODED BY TRANSMISSIVE PLASMIDS OF I AND N GROUPS

The work covers the cells of Escherichia colibacteria. The aim of the work is to identify the antirestrictive Ard proteins of transmissive plasmids pKM101 and ColIb-P9, to determine the nucleotide sequence of the genes coding them and to study the special features of their functioning in the E.colicells. The following investigation methods have been used: extraction and cleaning of plasmid DNA, restriction of DNA, cloning of DNA fragments into multicopeial vector, electrophoresis of DNA in agar gel and electrophoresis of proteins in polycrylamide gel, centrifuging in density gradient of chloride caesium, sequence process of DNA. The nucleotide sequences of two genes coding the antirestrictive functions of transmissive plasmids have been determined, and their products - antirestrictive Ard proteins - have been identified. The conservatism of primary structures in both antirestrictive proteins has been shown. The functional similarity of both antirestrictive proteins has been shown. The functional domen of Ard protein in the ColIb-P9 plasmid has been localized. The new mechanisms controlling the exchange process between bacteria have been studied that permits to understand better the ways and procedure of propagating resistance genes to the antibiotics in bacteria. The obtained results can be used at construction of the plasmid vectors for their use in the genic engineering and biotechnologyAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio