THE DEVELOPMENT METHODS OF THE DNA-HYBRIDIZATION AND POLYMERASE CHAIN REACTION (PCR) FOR THE IDENTIFICATION OF CLOSTRIDIUM BOTULINUM, TYPES A AND B

The purpose of the work: the development of the molecular-biological methods of identification. Obtained has been the probe for the DNA-hybridization, specific for the gene of neurotoxin C.botulinum of type A. Developed has been the specific and sensitive enough (5 ng DNA) method of the DNA-hybridization, allowing to reveal the presence of the neurotoxin gene, type A. Using the method of hybridization by Sauzern, first it has been shown, that two groups of strains, type A, having the different fields of DNA before the strating codon of the neurotoxin gene, exist. The PCR method for identification of C.botulinum, types A and B, has been developed; the high specificity and sensitivity (1 pg DNA) of this method have been shown. It has been shown, that the revealation of C.botulinum with use of method PCR can be performed in the whole number of food products, at this, in the same dilutions of samples, in which neurotoxin the biological breakdown on mice can be determinedAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio