Kultivierung von Schwammzellen in vitro als Basis fuer eine kontrollierte und effiziente Gewinnung von bioaktiven Substanzen Abschlussbericht

State of the Art: Sponges represent the richest source for natural products. Until recently, sponges could be obtained only by diving or collecting. No group succeeded to cultivate tissue fragments or even cells in vitro. Situation 1998: Until 1998 no method was available to establish a proliferating cell culture. Results: The breakthrough came after the application of molecular biological techniques. In our approach is was possible - for the first time - to cultivate in vitro of proliferating culture for more than 5 months; the cells were termed Primmorphes. Organisms: Sponge Suberites domuncula and Geodia cydonium. Both species grow in aquaria in Mainz all the year around. Marker for sponge cells: Suitable molecular tools have been worked out. Isolation of a cDNA-library from S. domuncula and other sponges. Isoaltion of suitable cDNAs: First cDNAs, which code for bioactive compounds have been isolated cDNAs. First bioactive proteins and low molecular weight compounds have been produced in culture. (orig.)Available from TIB Hannover: DtF QN1(84,25) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman