Entwicklung und Anwendung von Biosensoren in der Biotechnologie Nr. 5 Schlussbericht (Broschuere)

State of the art: Biosensores have been developed, but seldom applied in the biotechnology. Aim: Development of reliable biosensors and their application in the biotechnological practice. Method: Following sensors have been developed, tested and applied for process monitoring: pH- and fluoride sensitive field effect transistors (pH-FET, pF-FETs) and oxygen sensitive optodes. Heterogeneous affinity sensors were developed as well as applied for monitoring process variables in animal cell cultivations. Animal cell cultivation processes were optimized by these sensors. Knowledge-based fault detection and diagnosis systems were developed. Results: The optodes, PH-FETs, pF-FETs and affinity sensors were applied for monitoring of cultivations with bacteria, yeasts, fungi and animal cells with success. By means of the process monitoring the productivity of the blood factor AT III was improved. Another pH-(DLC)-, pO_2-, phosphate-, and H_2O_2-sensitive ISFET- and EIS-sensors were developed as well. Conclusions/applications: The biosensors developed in the frame of this project have been used for off-line and on-line process monitoring with success. They are suitable for bioprocess monitoring in laboratories and pilot plants. A company was founded for the commercialisation of the oxygen-optode. (orig.)Available from TIB Hannover: F97B689 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman