Klonierung von Matrixmetalloproteinasen und deren Expressionsmuster in der Maus AP-1 (Fos/Jun)-abhaengige Expression von Kollagenase I im Knochen

The extracellular matrix (ECM) functions as structural and mechanical support in the organism and additionally serves as a barrier between different cell types and organs. The maintenance of a dynamic equilibrium between synthesis and degradation of matrix components is therefore necessary. Matrix metalloproteinases (MMPs) are responsible for the specific degradation of the respective components of the ECM. These enzymes are thus involved in ECM remodelling. During embryonic processes, where an especially intense tissue remodelling occurs, a high expression of MMPs is expected. In cell culture, substrate specificities and expression of MMPs in different cell types and in response to extracellular stimuli, has been shown in human, rat and rabbit. Whether the in vitro properties of these enzymes are transferable to the in vivo situation has not been shown so far. In the work presented here, the cloning of the mouse specific MMPs stromelysin 1, stromelysin 2, collagenase I and the 72 kD collagenase IV was achieved. With these cDNA probes, the tissue specific expression of the four metalloproteinases could be demonstrated in the embryo as well as in the adult organism. (orig.)SIGLEAvailable from TIB Hannover: ZA 5141(5551B) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman