Klonierung und Expression von cGMP-gesteuerten Kanaelen aus Sehstaebchen und Zapfen der Huehnernetzhaut

The outer segments of rod and cone photoreceptor cells of the fowl (Gallus gallus gallus L.) retina contain cGMP-gated cation channels. Sequence analyses were carried out using a c DNA probe from the calf rod photoreceptor channel. The individual rod and cone channels could be identified by immunohistochemical investigations, sequence comparisons and Western-Blot-analyses. Subunits of both channels are shortened by a co- or post-translational, proteolytic modification in the N-terminal region. Electrophysiological channel properties were studied by heterologic expression in Xenopus oocytes. The rod channel exhibits a larger ligand sensitivity than the cone channel. Macroscopic flow and relative ion permeability are very similar in the expressed rod and cone photoreceptor cells. (WEN)SIGLEAvailable from TIB Hannover: RA 831(2871) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman