Untersuchung zur Wirkung ultravioletter Strahlung im Bereich zwischen 289 nm und 334 nm auf eukariontische Zellen Schlussbericht

In a study on the effect of monochromatic and polychromatic UV light on eukaryontic cells the following action spectra were determined: (I) Action spectras for the inactivation and mutation induction for the ouabain resistance in Chinese hamster cells between 254 nm and 313 nm, as well as under simulated sunlight, (II) action spectra for the induction of sister chromatid exchanges in Chinese hamster cells, (III) action spectra for the inactivation and mutation induction for cavanine resistance in yeast between 254 nm and 334 nm, as well as under simulated sunlight. Action spectra III closely resemble the absorption spectra of DNA, which is regarded as the primary chromophore. Discrepancies between calculated and measured actions of simulated sunlight point to an effective repair mechanism in yeast cells. Spectra II and I are interpreted by assuming another chromophore. In addition, in the case II an absorption by proteins is discussed. The effect of natural sunlight can be described as an additive action by light of the different wavelengths. An interaction with UV light cannot be excluded. (WEN)Available from TIB Hannover: F94B1847+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman