Physiologische und genetische Untersuchungen des Acetat-Stoffwechsels in Corynebacterium glutamicum

Based on the results of physiological and genetic characterizations a model of the regulation of the acetate metabolism in C. glutamicum has been developed. Corresponding to this model, during growth of C. glutamicum on acetate the intracellular acetyl-coenzyme A concentration is enhanced with the aid of the enzymes acetate kinase and phosphate transacetylase. As a consequence the gene expression for acetate kinase, phosphate transacetylase, isocitrate lyase and malate synthase is increased. At the same time a concerted inhibition of isocitrate dehydrogenase by glyoxylate (generated by the isocitrate lyase) plus oxalacetate leads to an increase of the intracellular isocitrate concentration and an intensified carbon flux in the glyoxylate cycle. (WEN)SIGLEAvailable from TIB Hannover: RA 831(2967) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman