Die Rolle des Homoeoboxgens goosecoid in der mesodermalen Differenzierung von embryonalen Stammzellen und der Teratokarzinomazellinie P19 der Maus

The homeobox gene goosecoid, originally identified in Xenopus, is expressed in the organizer or its equivalent during gastrulation in the frog, chick, zebrafish and mouse. To investigate the role of goosecoid in mouse development, embryonic stem cells were generated that stably overexpress the murine homologue of goosecoid under the control of the ubiquitin-promotor. These clones revealed a mesenchymal-like morphology distinct from wildtype ES-cells without any further differentiation steps. Vimentin, a marker gene for mesenchymal cells, is expressed in these cell clones. The morphological changes of these cells did not correlate with a reduction of the expression level of the cell adhesion molecule E-Cadherin. The goosecoid ES-clones express important organizer-specific genes like the neural inducers chordin and follistatin, the homebox gene Otx2 and the forkhead gene HNF3#beta#. Surprisingly, lim-1-mRA as well as the expression of gastrulation-associated gene Brachyury could not be detected in these cells. (orig.)Available from TIB Hannover: ZA 5141(6382) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman