Effect of 3-D Printable Scaffolds Hyperelastic Bone and Fluffy-PLG on Bone and Cartilage Differentiation of Mesenchymal Stem Cells

Ranging from cancerous infections to severe fractures, insults to osseous tissue has the propensity to yield significant debilitative effects in an individual. Current treatments present numerous risks such as donor site morbidity and infection. Thus, bone tissue engineering serves as a promising alternative to combat the shortcomings of allograft and xenograft implementation. Fluffy-PLG is an ultraporous (\u3e95%) polylacde-co-glycolide (PLG) copolymer that resembles cartilage tissue. Consequently, it can carry more mesenchymal stem cells (MSCs) and growth factors. With evident success in bone tissue regeneration, Hyperelastic Bone is a highly osteoinductive biomaterial consisting of 90% hydroxyapatite and 10% PLG. In this study both hyperelastic bone (HB) and fluffy-polylactide-co-glycolide (PLG) scaffolds were seeded with human urine progenitor stem cells to assess cell viability, proliferation, and differentiation in the presence of bone morphogenetic protein-9 (BMP-9) in vitro. The scaffolds’ ability to facilitate cell proliferation and osteogenesis were assessed by means of confocal fluorescence microscopy, alkaline phosphatase assays, dsDNA quantification, and expression of osteogenic genes