Modelling of Radiobiological Mechanisms in Cells and Tissues

Title: Modelling of radiobiological mechanisms in cells and tissues Author: Hana Hromcikova Department: Institute of Physics of the ASCR, v. v. i. Supervisor: RNDr. Milos Lokajicek, DrSc. Supervisor's e-mail address: lokaj@fzu.cz Abstract: The probabilistic two-stage model of cell inactivation enables to derive the characteristics of DNA damage induction and repair by an- alyzing published experimental survival data. To analyze in detail the cell inactivation effects of different ions at various energies, damage induction and repair processes in seA^eral hamster and human cell lines have been studied. Published survival data for Chinese hamster CHO- Kl cells and their radiosensitive mutants xrs5 irradiated by carbon ions and normal human nbroblasts irradiated by several light ions have been analyzed using the probabilistic two-stage model. The specialattention is paid to studying the cellular repair processes. The comparison with two common used radiobiological models, the Linear quadratic model and the Local Effect Model, will be presented, too. Keywords: irradiation, damage to DNA, cell survival curves, cell repair, radiobiological modelNazev: Modelovani radiobiologickeho mechanismu v bunkach a tkanich Autor: Hana Hrorncikova Oddeleni: Fyzikalni ustav AV CR3 v. v. i. Skolitel: RNDr. Milos Lokajicek, DrSc. E-mail skolitele: lokaj@fzu.cz Abstrakt: Pravdepodobnostnl dvoustupnovy model umozrLuje analyzou pub- likovanych experimentalnicli dat odvodit charakteristiky vzniku posko- zeni DNA a bunecne reparace. V praci bylo pomoci pravdepodobnos- tniho dvoustuprioveho modelu analyzovano nekolik sad experimental- nlch dat bunek cinskeho kfecka a lidskych koznlch fibroblastu. Velka pozornost byla venovana zejmena bunecne reparaci. Dale byly stu- dovany ucinky ruznych typu zafeni na bunky. V praci je take uvedeno srovnani rnezi pravdepodobnostnim dvoustupiiovym modelem a ostat- v radiobiologii pouzivanymi modely. Klicova slova: ozafeni, poskozeni DNA, bunecne krivky preziti, bunecna reparace, radiobiologicke modelovaniDepartment of Chemical Physics and OpticsKatedra chemické fyziky a optikyFaculty of Mathematics and PhysicsMatematicko-fyzikální fakult