Développement de vecteurs dérivés du virus de l'herpès simplex de type (HSV-1) pour le traitement des tumeurs cérébrales

HSV-1 derived vectors are promising tools for viral oncolysis or gene therapy by suicide gene of brain tumors. Attenuated recombinant vector are used for viral oncolysis. We have constructed and characterized a recombinant vector which carries a transgenic, biscistronic and auto-inductible expression unit. Defectif amplicon type vectors are tools with good potential for transfer of large size DNA in variety of cell types. We have constructed and developed an amplicon vector which carries the gene encoding the herpetic ICP0 protein. Human glioblastoma cells infected with this vector stop proliferating, resulting in cell death. No toxic effects were observed in non proliferaing infected cells.We have studied the expression of a second transgene carried by amplicon vectors and showed that though its expression is cell type dependent, simultaneous ICP0 expression could significantly increased itLYON1-BU.Sciences (692662101) / SudocSudocFranceF