Correlation between thermostability and stability of glycosidases in ionic liquid
- Ferdjani, Salim
- Ionita, Marina
- Roy, Bimalendu
- Dion, Michel
- Djeghaba, Zeineddine
- Rabiller, Claude
- Tellier, Charles
DOIAbstract
International audienceThe activity and stability of a β-glycosidase ( and two α-galactosidases ( and were studied in different hydrophilic ionic liquid (IL)/water ratios. For the ILs used, the glycosidases showed the best stability and activity in 1,3-dimethylimidazolium methyl sulfate [MMIM][MeSO] and 1,2,3-trimethylimidazolium methyl sulfate [TMIM][MeSO]. A close correlation was observed between the thermostability of the enzymes and their stability in IL media. At high IL concentration (80%), a time-dependent irreversible denaturing effect was observed on glycosidases while, at lower concentration (<30%), a reversible inactivation affecting mainly the was obtained. The results demonstrate that highly thermostable glycosidases are more su...
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