The fabrication, integration and application of multiphase microfluidic assay platforms for high throughput experimentation

This thesis describes novel research into the generation and manipulation of aqueous droplets within a number of multiphase microfluidic platforms. Specific techniques for the mixing, dilution and sorting of droplets are reported, and the development of droplet based microreactor devices for the generation of alginate gel beads described. The alginate beads have been applied for the encapsulation of both yeast and leukemia cells, as a potential platform for the study of the behaviour of regular assemblies of cells. further modification of the platform has also demonstrated the feasibility of double layer alginate encapsulation.EThOS - Electronic Theses Online ServiceGBUnited Kingdo