CDKA interacting proteins

Progression through the cell cycle relies on activity of cyclin-dependent kinases (CDKs) and their regulatory subunits, cyclins. CDKA and CDKB are the main components of the G2/M transition point in Arabidopsis thaliana. I aim to gain a better understanding of how mitotic kinases, CDKA and CDKB. mediate control of translation initiation and other biological processes throughout the cell cycle. To address this question, I used different approaches to identify interacting partners of CDKA and CDKB (and other cell cycle regulators). CDKA directly interacts with the complex that binds the 5' mRNA cap. In proliferating cells, CDKA associates with translation initiation factor eIF4A as demonstrated by reciprocal immonoprecipitation (Hutchins et al., 2004). To understand this interaction in greater depth, I used a pair-wise yeast two hybrid to test interactions between cell cycle regulators and translation initiation factors. I confirmed the association of translation initiation factors that is in agreement with the mechanism of cap complex assembly in animals and yeast and identified eIF4A and eIF4E as a putative substrates of both CDKA and CDKB2;1.EThOS - Electronic Theses Online ServiceGBUnited Kingdo