The role of sugars and sugar metabolism genes (sucrose synthase) in Arabidopsis thaliana seed development

Seed development in Arabidopsis thaliana, has been studied at several levels. However, little has been done to study the role of sugar metabolism genes in seed pod development in this species. As the fertilized egg progresses to a mature seed, the sugars composition during different stages of the developing changes. These changes are related to metabolic processes in the developing seeds, but also to the activity of sucrose- converting and transporting genes, active at the interphase between the maternal tissue and the endosperm. Sucrose synthase (SUS) is one of these genes; it catalyses the reversible reaction of sucrose breakdown in the presence of UDP to form fructose and UDP-glucose. In this study we looked at glucose, fructose and sucrose concentration at different time points during seed pod development. These changes in sugar concentrations were analysed in both Colombia wild type and WS (Wassilewskija) ecotypes. By comparison of the sugar composition of these ecotypes, and linking these data with phenotypic observations in both ecotypes during development, we are able to comment on the possible role of sugars in seed pod development. Also, the sugar composition of wild type seed pods were compared with those of Atsus mutant seed pods, and possible effects sucrose synthase mutations on the phenotype of the developing Arabidopsis thaliana seeds were analysed. The effect of sucrose synthase knockouts in developing seed pods were studied by comparing biochemical and phenotypic characteristics data of the Atsus mutants within Colombia wild type plants. Salk line plants were screened to identify plants carrying a homozygous insertion for T-DNA in five of the sucrose synthase genes. The developing seed pods of each of the homozygous mutants were characterized biochemically via High-Performance Anion-Exchange Chromatography (HPAEC). Furthermore, seed weight, number of seed per pod, germination rate and the morphological development of the embryo were closely analysed. The study found out that there were some biochemical effects of Atsus knockout mutants, and some phenotypic effects of Atsus knockout mutants on the developing seed pods. However, in general the effects were not as pronounced as those that were seen in maize seed, pea seed and potato tuber as a result of sucrose synthase knockout. The general pattern of glucose, fructose and sucrose were similar to the Colombia wild type, although in mature seed pods the sucrose levels in Atsus1, Atsus2, Atsus3 and Atsus6 were slightly, but significantly lower than in the Colombia wild type.EThOS - Electronic Theses Online ServiceGBUnited Kingdo