Role of HIF-1α in the resistance of Cancer Stem Cells to photon and carbon ion irradiations

International audienceHead-and-Neck-Squamous Cell Carcinoma (HNSCC) are resistant to standard treatments, partly due to Cancer Stem Cells (CSCs) localized in hypoxic niches. The protein HIF-1α (Hypoxia-Inducible Factor 1α) is considered as the major transcriptional regulator of the cellular response to oxygen homeostasis. Compared to X-rays, carbon ions relie on better ballistic properties, higher relative biological effectiveness and the absence of oxygen effect. HIF-1α is involved in the resistance to photons whereas its role in response to carbon ions remains unclear. This work aims at clarifying the role of HIF-1 in the response of CSCs to photon (250kV) and carbon ion (75Mev/n and 290Mev/μ) irradiations in normoxic or hypoxic (1% O2) conditions. For two HNSCC cell lines and their CSC subpopulation, in response to photons under hypoxia, an OER upper than 1.2 was associated with HIF-1α expression. This stabilization appears earlier in CSCs than in non-CSCs and is correlated with the variation of ROS levels, confirming the adaptive properties of CSCs to hypoxia. The diffuse ROS production by photons is concomitant with HIF-1α expression and essential to its activation. Compared with photons, the oxygen effect is canceled after carbon ion exposure (OER=1) and no stabilization of HIF-1α was observed in normoxia, probably due to the ROS localization in the track, insufficient to stabilize HIF-1α. Inhibition of HIF-1α with a siRNA leads to the decrease of HNSCC-CSC survival after both radiations in hypoxic conditions (OER