Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high-resolution flow cytometry
- Kormelink, Tom Groot
- Arkesteijn, Ger J A
- Nauwelaers, Frans A
- van den Engh, Ger
- Nolte - t Hoen, Esther
- Wauben, Marca H M
DOIAbstract
Submicron-sized vesicles released by cells are increasingly recognized for their role in intercellular communication and as biomarkers of disease. Methods for high-throughput, multi-parameter analysis of such extracellular vesicles (EVs) are crucial to further investigate their diversity and function. We recently developed a high-resolution flow cytometry-based method (using a modified BD Influx) for quantitative and qualitative analysis of EVs. The fact that the majority of EVs is <200 nm in size requires special attention with relation to specific conditions of the flow cytometer, as well as sample concentration and event rate. In this study, we investigated how (too) high particle concentrations affect high-resolution flow cytometry-base...
Add to ORKG