Two-dimensional electrophoresis of urinary mucopolysaccharides on cellulose acetate after f-cetylpyridiniumchloride (CPC) precipitation: A method suitable for the routine laboratory

A technique for two-dimensional electrophoresis of urinary mucopolysaccharides (MPS) is described. The method allows differentiation of a number of mucopolysaccharidoses and is suitable for application in the routine laboratory. This technique should be used to evaluate urines from patients who have a positive MPS-spot test and/or an increased urinary excretion of MPS-bound uronic acid. Urinary MPS excretion patterns are given from normal individuals, patients with the Sanfilippo syndrome and the Hunter syndrome