The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis

  • Smith, Stephanie
  • Zhu, Shanshuo
  • Joos, Lisa
  • Roberts, Ianto
  • Nikonorova, Natalia
  • Vu, Lam Dai
  • Stes, Elisabeth
  • Cho, Hyunwoo
  • Larrieu, Antoine
  • Xuan, Wei
  • Goodall, Benjamin
  • van de Cotte, Brigitte
  • Waite, Jessic Marie
  • Rigal, Adeline
  • Harborough, Sigurd Ramans
  • Persiau, Geert
  • Vanneste, Steffen
  • Kirschner, Gwendolyn K.
  • Vandermarliere, Elien
  • Martens, Lennart
  • Stahl, Yvonne
  • Audenaert, Dominique
  • Friml, Jiri
  • Felix, Georg
  • Simon, Ruediger
  • Bennett, Malcolm J.
  • Bishopp, Anthony
  • De Jaeger, Geert
  • Ljung, Karin
  • Kepinski, Stefan
  • Robert, Stephanie
  • Nemhauser, Jennifer
  • Hwang, Ildoo
  • Gevaert, Kris
  • Beeckman, Tom
  • De Smet, Ive
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Publication date
January 2020
Language
English

Abstract

Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabi-lizes AUX/IAA transcriptional re...

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