Effets oestrogéniques chez des cyprinidés

[Departement_IRSTEA]GMA [TR1_IRSTEA]12 - MILEAU / EXPERLaboratory and field studies were carried out in order to develop and validate biological methods to detect and quantify estrogenic effects on fish. Experiments were conducted on cyprinid species : chub (Leuciscus cephalus), gudgeon (Gobio gobio) and zebrafish (Danio rerio). Vitellogenin (Vtg) induction was quantified by immunodetection method (ELISA) using anti-carp antibody (Brunel University) and purified carp, gudgeon and chub Vtg proteins. In parallel, histological studies on male gonads were also performed on the three species. Preliminary field studies have been carried out on the chub and will be complemented in the near future with other field samplings. In the laboratory, experiments are being performed in zebrafish to investigate effects of exposure to estrogens on Vtg induction, gonad development and reproductive performance (egg number).Des études de terrain et de laboratoire ont été effectuées sur le chevaine et le poisson zèbre. Des mesures de vitellogénine (ELISA) et d'altérations histologiques des gonades mâles ont été réalisées