Response of Wheat Seedlings to Water Loss Caused by Osmoticum

Kao rezultat klimatskih promjena suša je postala svjetski problem koji ograničava proizvodnju pšenice. Posebno osjetljive na sušu su mlade sadnice. Kako bi stvorile otpornost i obranile se od oksidativnog stresa uzrokovanog sušom, biljke su razvile niz adaptivnih i obrambenih mehanizama. Apcizinska kiselina ima vrlo vaţnu ulogu u borbi biljaka protiv stresa. Za cjelovit razvoj i optimalan prinos presudno je znati kako odreĎeni genotip reagira na sušu. Cilj ovog istraţivanja bio je ispitati postotak klijavosti, izdanke i korijenje tijekom šest dana u kontroliranim uvjetima. Za stvaranje osmotskog stresa koristili smo polietilenglikol (PEG-6000). To je polimer koji u vodenoj otopini ne stvara ione i koji ne moţe lako prodrijeti u biljku, pa se često koristi u pokusima vezanim za sušu. U ovom istraţivanju korištene su tri koncentracije PEG-a (15%, 20% i 30%). Sjeme je počelo klijati nakon drugog dana istraţivanja. Najviša koncentracija PEG-a (30%) imala je najjači inhibitorni učinak na klijanje u usporedbi s 15% i 20% koncentracija PEG-a tijekom svih šest dana. Duljina korijena pokazala je smanjenje za 20 i 30% koncentracije PEG u usporedbi s kontrolom. Najviša koncentracija PEG-a (30%) imala je najviše štetnog utjecaja na klijavost, izdanak i duljinu korijena. Duljina korijena nije se značajno promijenila izmeĎu biljaka koje su rasle na 15 i 20% PEG te 20 i 30% PEG što govori kako korijen uspješnije tolerira sušu od izdanka.As a result of climate changes, drought has become a worldwide problem limiting wheat production. Young seedlings are especially susceptible to drought. To create resistance and defence against oxidative stress caused by drought, plants have developed several adaptive and defensive mechanisms. Abscisic acid has an important role to fight against stress. Knowing how a particular genotype responds to drought is crucial for full development and optimum yield. The purpose of this study was to examine the percentage of germination, shoots, and roots over six days in controlled conditions. To create osmotic stress we used polyethylene glycol (PEG-6000) as osmoticum. It is a non-ionic water-soluble polymer that cannot easily penetrate into the plant so it is often used in drought studies. In this study, three PEG levels (15%, 20%, and 30%) were used. Seeds were started to germinate after the second day of the experiment. The highest PEG concentration (30%) had the strongest inhibitory effect on germination compared to 15% and 20% PEG concentrations during all six days. Root length exhibited reduction at 20 and 30% PEG treatments compared to control. The highest PEG concentration (30%) had the most damaging effect on germination, shoot, and root lengths. Root length did not change significantly between plants that grew to 15 and 20% PEG and 20 and 30% PEG, respectively, indicating that the root more successfully tolerated drought than shoots