Migration of phthalates from plastic tank to vegetable oil as a part of feeding mixtures used for chicken broilers fattening

The concentrations of phthalic acid esters (PAEs) as di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) were measured in samples of rapeseed oil, which was used as a feed. First samples were collected during the production process and second after the storage in plastic tank (21 days). The results of measurements are that there is 2.93-10.10 mg PAEs.kg-1 in the oil before storage and 22.73-61.55 mg PAEs.kg-1 after storage. For the monitoring of distribution and accumulation of PAEs in animal tissues and organs (muscles, adipose tissue, skin and liver) broiler chicks ROSS 308 were used. The chicks were divided into 4 groups (50 chicks each). All the chicks were fed by commercial diets (complete feed, KKS) for broiler chicks (starter – BR1; grower – BR2 and finisher – BR3). The experimental diets were supplemented with vegetable oil (RO) with low (group N) or high (group V) phthalate content, or animal fat with high phthalate content (group Z). Neither the control diets (K) nor the grower (BR1) diets contained vegetable oil or animal fat. DBP and DEHP were found in all tissues of all chicks. The highest concentration of DBP of 1.28 1.00 mg.kg-1 of fresh sample (an average value from 8 chicks) was determined in the adipose tissue of V chicks. The highest concentration of DEHP of 3.27 2.87 mg.kg-1 of fresh sample (average of 8 chicks) was also determined in the V group