Antibiotic resistance profile of gram negative uropathogen isolated from a tertiary care center in Kolkata

Background:Urinary tract infections are one of the most common infectious disease in India. Resistance pattern of common antimicrobials to uro-pathogens are changed drastically over last few years due to widespread use of antibiotics. Aim and Objectives: The aim of this study is to determine the bacterial etiology of UTI and the antimicrobial resistance pattern of gram-negative uro-pathogens isolated among the patients attending in a tertiary care hospital in Kolkata.Material and methods:The study was conducted to determine the distribution and antibiotic sensitivity pattern of gram-negative uro-pathogen in the Department of Microbiology, NRSMC&H, Kolkata from March 2019 to February 2020.Freshly voided clean catch midstream urine specimens were collected from suspected hospitalized and OPD patients and processed bacteriologically as per standard procedures. Antimicrobial susceptibility and resistance was detected phenotypically as per CLSI guidelines. Results:From 5930 urine specimens, 36.42% had positive results for bacterial cultures. Growth of gram negative uro-pathogens was 80.32%. Female (65.37%) was outnumbered and most of the cases belongs to the age group of 30-50 years (42.60%). E. coli was the most prevalent uro-pathogen (61.78%). High degree of resistance was noted in Klebsiella spp.Most of the isolates of our study were ESBL producer (68.54%).Conclusion:This study reinforced that Treatment for UTIs should always be considered based on current local antimicrobial susceptibility patterns of uro-pathogens to reduce therapeutic failures and prevent antibiotic misuse