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Carbon neutral bio-oil is needed to replace liquid fossil fuels in order to reduce CO2 emissions and global warming. However, the quality of bio-oil produced from biomass contains too much sugar, which is undesirable. Investigating a new method to reduce sugar concentration by changing the ratio between cellulose and catalyst is necessary because other methods all require additional costs. In this study, the Ni2Fe3 and cellulose have been chosen as the model catalyst and model biomass compound, respectively. Five different ratios have been chosen for investigation, 4:1, 2:1, 1:1, 1:2 and 1:4 (weight ratio= cellulose: catalyst) The cellulose pyrolysis experiments results show the bio-oil yield increases as the catalyst amount or ratio is increased initially and then decreased. The 2:1, 1:1, 1:2 shows the highest yields among the five different ratios. From GC-MS analysis, the sugar reduction increases initially and then decreases. Based on both results, the best cellulose/catalyst ratio was 2:1 or 1:1, which is attributed to optimized heat conduction