Efektivitas Salep Ekstrak Etanol 70% Daun Sirih terhadap Epitelisasi Luka Tikus Putih

Daun sirih (Piper betle Linn.) dapat berfungsi sebagai antiseptik, antioksidan, antibakteri, antiinflamasi, dan astringen sehingga berpotensi mempercepat proses penyembuhan luka. Tujuan penelitian ini untuk mengetahui efek salep ekstrak etanol 70% daun sirih terhadap fase epitelisasi tikus putih jantan galur wistar yang mengalami luka di area punggung. Penelitian dilakukan di Laboratorium Farmakologi dan Farmasi Klinis Fakultas Kedokteran Universitas Tanjungpura pada bulan November 2018 - Januari 2019 menggunakan desain posttest only controlled group design. Penelitian menggunakan 30 tikus yang dibagi 2 kelompok terdiri atas pengamatan hari ke-7 dan hari ke-14. Setiap kelompok pengamatan terdiri atas 5 kelompok, kelompok kontrol negatif (K-), kelompok kontrol positif (K+), kelompok sirih 7,5% (PI), kelompok sirih 15% (PII), dan kelompok sirih 30% (PIII). Setelah perlukaan, semua kelompok perlakuan dioleskan salep ekstrak etanol 70% daun sirih 2 kali sehari, pagi dan sore. Penilaian epitel menggunakan preparat histopatologi dengan pewarnaan hematoxylin-eosin. Analisis data menggunakan program ImageJ dan IBM SPSS v.24. Luka kelompok salep PII hari ke-7 mengalami epitelisasi luka paling baik dibandingkan kelompok lainnya, Pengukuran jarak antara folikel rambut pertama di tepi luka dan jarak antara dua tepi epitel luka pada hari ke-7 maupun hari ke-14 tidak berbeda signifikan pada semua kelompok. Disimpulkan salep ekstrak etanol 70% daun sirih 15% mempunyai daya penyembuhan luka yang baik pada fase epitelisasi hari ke-7. Effectiveness of Ointment 70% Ethanol Extract Betel Leaves against Wound Epitelialization on Rats  Betel leaf (Piper betle Linn.) can function as an antiseptic, antioxidant, antibacterial, anti-inflammatory, and astringent so that it has the potential to accelerate the wound healing process. The purpose of this study was to determine the effect of 70% betel leaf ethanol extract ointment on the epithelialization phase of Wistar male white rats with wounds in the back area. The research was conducted at the Laboratory of Pharmacology and Clinical Pharmacy, Faculty of Medicine, Tanjungpura University in November 2018 - January 2019 using a posttest only controlled group design. The study used 30 rats divided into 2 groups consisting of observations on the 7th-day group and the 14th-day group. Each observation group consisted of 5 groups, a negative control group (K-), a positive control group (K +), a 7.5% betel group (PI), a 15% betel group (PII), and a 30% betel group (PIII). After wounding, all treatment groups were applied with 70% ethanol extract ointment of betel leaf 2 times a day, morning and evening. The epithelial assessment was using histopathological preparations with hematoxylineosin staining. Data analysis using ImageJ and IBM SPSS v.24 programs. Wounds of the PII ointment group on day 7 experienced the best wound epithelialization compared to other groups. Measurement of the distance between the first hair follicles at the wound edge and the distance between the two wound epithelial edges on day 7 and day 14 did not differ significantly in all groups. It was concluded that the 70% ethanol extract ointment of 15% betel leaf had a better wound healing process on the 7th day of the epithelialization phase