Biochemical alterations in total proteins and related enzymes in tissues of Cyprinus carpio (L.) during sublethal exposure to karanjin based biopesticide Derisom

125-131Excess usage of synthetic pesticides in agricultural fields perculates down the ecosystem to other livestock and fishes and thereby pose threat to humans. This has lead to prominence of bio pesticides which have always been thought to be harmless to the non-target organisms. However, literature on the safety of these biopesticides is limited. In this context, here, we studied the effect of Karanjin based botanical biopesticide Derisom on the biochemical parameters in various tissues of a fresh water fish species. We selected the edible common carp Cyprinus carpio (L.) for the study as it is an important protein food source for humans. The effect of the biopesticide - Derisom on certain metabolites and enzymes of protein metabolism were evaluated in gill, liver, kidney and the muscle of C. carpio during sublethal toxicity exposure of 21 days. About 0.28 ppm was taken as the sublethal value. The organs were taken from the exposed and control groups at the end of 1h, 7, 14 and 21 days, and were used for estimation of total proteins, free amino acids, glutamate dehydrogenase (GDH), alanine amino transferase (ALAT) and aspartate aminotransferase (AAT). All the four organs showed significant difference between control and exposed groups in all the estimated parameters on all the days of exposure. The present study considers the above biochemical parameters as an important biomarker in determining the level of toxicity caused by the biopesticide Derisom