The prevalence of Salmonella, Campylobacter and VTEC in pig farms

Four different pig farms were sampled for the prevalence of Salmonella, Campylobacter and VTEC. From a total of 215 rectal samples of individual pigs, 15 rectal samples taken from animals at the same farm were positive for Salmonella. The Salmonella status of the pigs at this farm differed from one age group to another. S. Typhimurium was isolated from all the rectal samples and S. Typhimurium and S. Schwarzengrund were isolated from the environment. On two other farms Salmonella was only present in the environment with S. London and S. Typhimurium as serotypes. With cut-off value %OD\u3e 40 in the ELISA we found a good correlation with the Salmonella status of the farm. The presence of Campylobacter was tested in 150 rectal swabs, 51 of these, spread over the four farms, turned out positive. All the strains were identified as Campylobacter coli by a species-specific PCR. To determine if pigs are a reservoir of VTEC a total of 289 samples were screened for the presence of VTEC and 54 strains were isolated that each carried one virulence gene. Thirty-one strains carried the vt2e variant of the vt2 gene, four strains harboured the hly A gene and 19 the eaeA gene